Designed to stand alone or for use with other materials, each manual offers hands-on experience with anatomical structures and physiological concepts to aid in mastery of the subject. The new PhysioExVersion 5. The scientific method, metrics, the human body, the microscope, the cell, histology, the integumentary system and body membranes, plus coverage of the skeletal, muscular, nervous, endocrine, circulatory, respiratory, digestive, urinary and reproductive systems, development, heredity, surface anatomy, dissection exercises, PhysioEx Computer Simulations, PhysioEx Review Sheets, PhysioEx Histology Review Supplement, Histology Atlas, Human Anatomy Atlas, Review Sheets.
Open in a separate window Figure 6. Cut the cuticle on the ventral half of the propodite to expose the closer muscle so it can be removed. Click here to view larger image. Exchange the saline with fresh cooled saline throughout the dissection process to keep the neurons alive.
For further dissection, place the preparation dish under a dissecting microscope and use fiber-optic illumination. Carefully cut the closer tendon from its attachment to the dactyl using sharp-pointed medium-size scissors.
Be very careful not to disturb the branches to the opener muscle from the main leg nerve which should be clearly visible. Remove and discard the closer muscle and tendon. Make a hole in the dactyl with a dissection pin.
This hole will be used later to hook the metal pin on the tension force transducer, but it is necessary to make it at this stage of the procedure.
Locate the nerve branch that projects to the opener muscle and apodeme in the distal region of the opener muscle. Carefully probe the nerve with the fire-polished glass tool.
Observe the tension nerve that arises from the distal end of the apodeme and proceeds to the motor nerve bundle. To detect neural activity, fill a suction electrode with crab saline and draw the cut end of the opener tension nerve into the electrode.
Ensure that the suction electrode fits tightly on the nerve. Examine for the neural correlate of passive tension on the opener apodeme.
While recording electrical activity from the tension nerve, rotate the dactyl rapidly into an extended joint i. Next, test active force development in relation to stimulation frequency.
Firmly attach a metal hook so that the tip of the hook goes through a small hole in the dactyl. Attach the other end of the metal hook to the force transducer. Now stimulate the motor nerve at Hz for msec and measure the force as well as the firing frequency of the tension nerve.
Place the joint into a fully extended position so that the opener muscle fibers are flaccid. Stimulate the motor nerve at Hz for msec and measure the force as well as the firing frequency of the tension nerve. In this position the muscle fibers of the opener are fully stretched.
There may be some force measured by the transducer due to this passive stretch of the muscle.Start studying Exercise 16 Skeletal Muscle Physiology.
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Skeletal Muscle Physiology.